Paramaiswari, Wisiva Tofriska and Sidik, Nurma Sumar and Khoeri, Miftahuddin Majid and Tafroji, Wisnu and Said, Wahyu Finasari and Safari, Dodi and Falkinham, Joseph (2021) Isolation and Identification of Optochin-Resistant Viridans Group Streptococci from the Sputum Samples of Adult Patients in Jakarta, Indonesia. International Journal of Microbiology, 2021. pp. 1-5. ISSN 1687-918X
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Abstract
Aim. To investigate optochin-resistant viridans group streptococci (VGS) strains isolated from the sputum sample of adult patients with different clinical symptoms. Materials and Methods. Optochin-resistant VGS isolates were identified by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS). recA sequencing was used to confirm identified isolates at the genus level by MALDI-TOF MS. Finding. We identified 79% of tested isolates (148/187) at the species-level identification using the MALDI-TOF MS tool. We identified that the most common species isolated from sputum specimens were S. oralis (44.9%) followed by S. mitis (25.7%), S. infantis (9.1%), S. parasanguinis (7.5%), S. peroris (3.7%), S. anginosus (2.7%), and S. sanguinis (2.1%). Discussion. The S. oralis strains were majority of optochin-resistant VGS isolates obtained from sputum of adult patients in Jakarta, Indonesia. MALDI-TOF MS showed potential for the rapid identification tool to identify optochin-resistant VGS isolates. Although there were discrepancies in identifying isolates at the genus/species level, the performance could be improved by expanding its database.
Item Type: | Article |
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Subjects: | EP Archives > Biological Science |
Depositing User: | Managing Editor |
Date Deposited: | 13 Mar 2023 06:17 |
Last Modified: | 04 Jul 2024 06:47 |
URI: | http://research.send4journal.com/id/eprint/337 |