Serum Amino Transaminases Activities and De Ritis Ratio amongst Apparently Healthy Secretors and Non-Secretors of ABH Substances Dwelling in Uyo Urban, Akwa Ibom State, Nigeria

Aims: The current study was designed to determine a local reference range of serum Alanine (ALT) and Aspartate (AST) activities and De Ritis ratio amongst apparently healthy secretors and non-secretors of ABH substances within Uyo, Akwa Ibom State, Nigeria, aged between 20 to 60 years for both genders.

Study Design: The current study design was experimental and simple randomized sampling techniques was employed.

Place: The current study was carried out in Uyo, Akwa Ibom, State, Nigeria.

Duration of the Study: From 12th June to 6th December 2017.

Methodology: About 2ml of saliva and 5ml of Blood samples were collected from 400 subjects by standard procedures and both serum ALT and AST activities were determined by a spectrophotometric techniques using commercial kit reagent produced by Sigma-Aldrich Cooperation Limited Liability Company, USA while ABH secretor status determined by Haemagglutination Inhibition Method using Anti-H -Lectin kits produced by BIOTEC Laboratories Limited, Suffolk, United Kingdom.

Results: Statistically Chi-Squared (X2) test shows significant differences (P <0.05) between the means serum AST and ALT levels according to gender ((X2) = 4.99, P =0.025). No correlation found between serum ALT and AST activities with age and genders (r=0). De Ritis ratio = <1 according to gender, with no significant difference (P >0.05) between genders (t=2.44, P =0.1238,). Significant difference (P <0.05) between activities of ALT and AST in ABH secretor and non-secretors and sexes ((X2) =5.1, P =0.0239,). Chi Squared shows no significant differences in the mean prevalence rate ratio between gender (X2) = 3.2, df = 1, P = 0.07364, P >0.05). The prevalence rate ratio of ABH secretors and non-secretors was 4:1, according to ages and there was a significant difference (P <0.05) between male and female genders (t=2.44, P =0.1238,)

Conclusion: AST and ALT references ranges in male ABH secretors subjects were higher than in female non-secretors subjects respectively.