Tissue Culture Studies for Shoot Multiplication and Herbicide Tolerance in Certain Grain Legumes

Plant tissue culture and Biotechnology is considered in a wide sense which comprises the various culture methods of plant organs and explants to facilitate experimental approaches with a large objective of developmental biology in grain legumes for crop modification. Studies were made to establish the static cultures using various seedling explants of Vigna, Cicer, Glycine and Cymopsis to investigate the morphogenic potential and selection against the herbicides and antimetobolites. High efficiency of callus initiation and somatic embryogenesis were observed in Glycine max followed by Vigna. The recalcitrant species Cyamopsis not responded well under in vitro experimental conditions for both multiple shoot development from cotyledons, the high efficiency of multiple shoot induction observed cotyledonary cultures of Glycine max which facilitate genetic transformation for production of transgenic plants. Embryogenic suspension cultures of Vigna and Glycine selected against the Atrazine and Glyphosate 20mM for Herbicide tolerance.