Somatic Embryogenesis from Nucellar Tissues of Mango (Mangifera indica Linn.)

Callus induction and somatic embryogenesis were obtained from de-embryonated nucellar tissues of young mango (Mangifera indica Linn.) cultivar �Natumanga� fruits on MS medium supplemented with 4.52 �M 2,4-D. Mostly globular/heart shaped embryos from each half of the nucellus was noticed within a period of 80 days. Embryos transferred to M S medium with 2.26 �M 2,4-D showed secondary asynchronous embryogenesis resulting in globular, heart shaped and cotyledonary stages of development. Histological studies were conducted to confirm the developmental stages of embryos. Embryo germination was inhibited due to the abundance of phenolics oozed out to the medium.