Experimental Study on the Development of Paper Biosensor for the Detection of Phenol from Industrial Effluents Using Bioconjugate of Tyr-AuNps Mediated by Novel Isolate Streptomyces tuirus DBZ39

In the present study, paper biosensor was developed using Tyr-AuNps bioconjugate produced by
Streptomyces for the detection of phenol from the effluent of wine, paper and plastic industries.
Among three filter papers assessed, Whatman No.2 filter paper was proved to be the best paper
base for the development of biosensor. Tyrosinase and gold nanoparticles being produced by a
single novel isolate Streptomyces tuirus DBZ39, proved to be efficient bioconjugate for the detection
of phenol constituents, due to its biocompatibility. The substrate specific catalytic activity of the
tyrosinase and unique Surface Plasmon Resonance attribute of gold nanoparticles are the cause for
efficient detection of phenol constituents from the effluent of wine, paper and plastic industries in 3
min. The different types and quantity of phenolic constituents in various industrial effluents, such as
phenol in wine, dopamine in paper and catechol in plastic effluents were accurately detected by the
bioconjugate. The efficacy of tyrosinase in the detection of phenol constituents was expected to be
enhanced by the gold nanoparticles because of their electron, optical and magnetic properties. This
novel paper strip biosensor could be cost effective and efficient means of future devices for the
detection of phenolic pollutants from any environmental samples.