An Experimental analysis of Hepatotoxic-Induced Physiological Alterations and the Protective Role of Cleome viscosa Linn Seed Extract in Animals

The goal of the current investigation was to evaluate the antioxidant and hepatoprotective properties of seed extracts from Cleome viscosa Linn. (Capparaceae). Liver ailment is a key public health menace, principally in developing nations. Quite a lot of medicinal florae have been identified to have liver shielding activities. C. viscosa seed ethanol extract underwent phytochemical screening. The 2,2-diphenyl-2-pic- rylhydrazyl (DPPH) test technique was used to measure the crude seed extract of the plant's ability to scavenge free radicals. The genomic DNA nicking assay extract was used to test the crude seed extract's capacity to protect against DNA damage. Based on CCl4-induced liver damage in Wister albino rats, and the crude seed extract of the plant was tested for hepatoprotective efficacy. Serum biomarkers (aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), and total protein (TP)) were evaluated to find out the efect. Histopathological scrutiny was also carried out for all groups of rats to further confirm the discoveries. The phytochemical screening was positive for alkaloids, flavonoids, saponins, steroids, terpenes, tannins, and phenolic compounds in the seed extract. The antioxidant assay revealed that the ethanol crude extract of C. viscosa exhibited free radical scavenging activity with IC50 value of 17.82 ± 0.32 g/mL, and this was further confirmed by the DNA damage protection activity. Rats pretreated with the crude extract of C. viscosa had their ALP considerably lowered (p < 0.05). Histopathological tests verified the seed extract's hepatoprotective effects. According to this investigation, the crude seed extract exhibited antioxidant and hepatoprotective properties. Because of this, it may be beneficial in the future to take C. viscosa seed extract orally as an additional natural medicine to guard against liver failure-mediated inhibitory effects on reproductive function.