Long-Term Consumption of Anthocyanin-Rich Fruit Juice: Impact on Gut Microbiota and Antioxidant Markers in Lymphocytes of Healthy Males

Groh, Isabel Anna Maria and Riva, Alessandra and Braun, Dominik and Sutherland, Heidi G. and Williams, Owen and Bakuradze, Tamara and Pahlke, Gudrun and Richling, Elke and Haupt, Larisa M. and Griffiths, Lyn R. and Berry, David and Marko, Doris (2020) Long-Term Consumption of Anthocyanin-Rich Fruit Juice: Impact on Gut Microbiota and Antioxidant Markers in Lymphocytes of Healthy Males. Antioxidants, 10 (1). p. 27. ISSN 2076-3921

[thumbnail of antioxidants-10-00027-v2.pdf] Text
antioxidants-10-00027-v2.pdf - Published Version

Download (4MB)

Abstract

Polyphenols are considered protective against diseases associated with oxidative stress. Short-term intake of an anthocyanin-rich fruit juice resulted in significantly reduced deoxyribonucleic acid (DNA) strand-breaks in peripheral blood lymphocytes (PBLs) and affected antioxidant markers in healthy volunteers. Consequently, effects of long-term consumption of fruit juice are of particular interest. In focus was the impact on nuclear factor erythroid 2 (NFE2)-related factor 2 (Nrf2), the Nrf2-regulated genes NAD(P)H quinone oxidoreductase 1 (NQO-1) and heme oxygenase 1 (HO-1) as well as effects on the gut microbiota. In a nine-week placebo-controlled intervention trial with 57 healthy male volunteers, consumption of anthocyanin-rich juice significantly increased NQO-1 and HO-1 transcript levels in PBLs compared to a placebo beverage as measured by real-time polymerase chain reaction (PCR). Three Nrf2-promotor single nucleotide polymorphisms (SNPs), analyzed by pyrosequencing, indicated an association between individual Nrf2 transcript levels and genotype. Moreover, the Nrf2 genotype appeared to correlate with the presence of specific microbial organisms identified by 16S-PCR and classified as Spirochaetaceae. Furthermore, the microbial community was significantly affected by the duration of juice consumption and intake of juice itself. Taken together, long-term consumption of anthocyanin-rich fruit juice affected Nrf2-dependent transcription in PBLs, indicating systemic effects. Individual Nrf2 genotypes may influence the antioxidant response, thus requiring consideration in future intervention studies focusing on the Nrf2 pathway. Anthocyanin-rich fruit juice had an extensive impact on the gut microbiota.

Item Type: Article
Subjects: EP Archives > Agricultural and Food Science
Depositing User: Managing Editor
Date Deposited: 04 Jul 2023 03:59
Last Modified: 07 Oct 2023 09:28
URI: http://research.send4journal.com/id/eprint/2471

Actions (login required)

View Item
View Item