Phytochemical Analysis, Antimicrobial and Antioxidant Activity of Clitoria ternatea leaves of Blue and White Flower

The present study was conducted to screen the phytochemical constituents, identifying the compounds present by Gas Chromatography-Mass Spectrometric analysis (GC-MS), functional groups present by Fourier Transform Infrared Spectrophotometer (FT- IR) in the ethyl acetate leaf extract of Clitoria ternatea white flower, explicit the antibacterial activity, antioxidant assay of using methanol and ethyl acetate leaves extract of Clitoria ternatea blue and white flower. Clitoria ternatea leaves of blue flower possess 7.5% of total ash whereas leaves of white flower possess 8.5%. The phytochemical analysis revealed that leaves of blue flower contain alkaloids, flavonoids, phenols, terpenoids, glycosides, coumarins, catechol, quinines, gum and mucilage while, in leaves of white flower had alkaloids, glycosides, catechol, gum and mucilage were present. A total of 8 compounds such as 1- Decanol, 2- ethyl, 1- Eicosanol, Sulphurous acid octadecyl 2- propyl ester, Eicosanoic acid, L- (+)- ascorbic acid 2,6- dihexadecanoate, Sulphurous acid, pentadecyl 2-propyl ester, Oleic acid, 1- Hexyl-2 nitrocyclohexane were detected in the ethyl acetate leaves extract of C. ternatea white flower using GC-MS. FT-IR analysis of the same had characteristic bands at 2983.88 cm¯ 1 indicating the presence of C-H stretching. The ethyl acetate leaves extract of white flower C. ternatea had antibacterial and antifungal activity against E. coli, S. aureus, K. pneumoniae, P. aeruginosa, P. mirabilis, C. albicans, C. tropicalis, C. kruzi. The total phenolic content of methanolic leaf extract of C. ternatea blue flower was 25 mg Gallic Acid Equivalent/ g Dry Weight (mg GAE / g DW), and leaf of white flower was 18 mg GAE / g DW. The total flavonoid content of methanolic leaf extract of C. ternatea blue flower was 10 mg Quercetin Equivalent/g Dry Weight (mg QE / g DW), and leaf of white flower was 5 mg QE/g DW. Maximum scavenging activity of 86.6% was observed in leaf ethyl acetate extract of blue flower (DPPH assay). Maximum scavenging activity of 82% (H2O2 assay) and 95% (total antioxidant) was observed in the ethyl acetate leaf extract of white flower.